The Tenzer Lab focuses on the development of label-free, quantitative proteomics methods and workflows and harbours the "Core Facility for Mass Spectrometry" (CFMS) at the University Medical Center Mainz. To enable the reproducible proteomic analysis of large clinical cohorts, we develop automated high-throughput sample processing workflows on a Beckman Biomek i7 robotic platform. Using several latest generation LC-MS instrument platforms, including Bruker TIMS-TOF Pro 2 systems and a FAIMS-equipped Thermo Exploris 480 system, we explore the benefits of ion mobility separations for quantitative proteomics and immunopeptidomics. Besides the development and application of data independent acquisition workflows (UDMSE, diaPASEF), we pioneered benchmarking methods and software solutions for quantitative proteomics (LFQBench) and comprehensive raw data access (openTIMS, TIMSpy).
Within the DIASyM research core, we optimize and standardize workflows for sample processing, LC-MS analysis and subsequent data processing to achieve an optimal coverage of the plasma proteome in patient and respective population control samples. Together with our partners in DIASyM, we will integrate multilevel OMICs data sets to stratify and phenotype patients suffering from heart failure syndrome.